Samsung sank more than 2 per cent, while SK Hynix stock was more than 4 per cent lower in morning trading.
The pair had operated in China under regulations that allow them to import chipmaking equipment without applying for a new licence each time.
They now have 120 days until the waiver expires, according to an announcement in the US Federal Register. Companies can seek licences to continue operations.
The Trump administration’s move would revise what is known as the validated end user, or VEU, rules, handicapping the ability to make chips in China and jeopardising Beijing’s access to certain technologies.